Antibodies © Adobe Stock
Anaphylactic shock, an exacerbated allergic reaction that can prove fatal, is sometimes caused by the use of drugs during surgery. In most of these extreme reactions, evidence can be provided that patients have anti-drug antibodies of the IgE class. In 10 to 20% of anaphylactic cases evidence for the involvement of anti-drug IgE is lacking. Anti-drug IgE enable activation of mast cells and basophils that release histamine, a potent mediator involved in anaphylaxis. Teams from the Institut Pasteur, Inserm, the Paris Public Hospital Network (AP-HP), the CNRS, Paris-Sud University and Sorbonne University have successfully identified a new pathological mechanism responsible for these previously unexplained cases, involving neutrophils activated by antibodies of the IgG class. These findings, published on July 10 in the journal Science Translational Medicine, will help improve diagnosis and treatment for patients with this type of shock.
Anaphylaxis is a hyperacute allergic reaction caused by an inappropriate immune response following the introduction of a usually harmless antigen into the body. When this antigen binds to antibodies already in the body, it triggers the secretion of large quantities of potent vasodilating mediators, sending the patient into a state of shock that may result in multiple organ failure and even death.
Although it was already known that IgE antibodies could cause anaphylaxis, teams from the Institut Pasteur, Inserm, the Paris Public Hospital Network (AP-HP), the CNRS, Paris-Sud University and Sorbonne University have demonstrated in a clinical study that IgG antibodies can also be involved in drug-induced anaphylactic shocks. This unexpected role of IgG antibodies had already been identified in mice in 2011 by some of the same authors.
This multicenter study known as “NASA”, led by Bichat Hospital (part of the Paris Public Hospital Network), was launched in 2012 by a consortium of scientists, clinical biologists and anesthetists. The consortium monitored 86 patients with perioperative anaphylactic shock and 86 control patients in 11 hospitals in the Greater Paris area, coordinated at Bichat Hospital by immunologist Professor Sylvie Chollet-Martin (Paris-Sud University) and anesthetist Professor Dan Longrois. Blood samples were taken as soon as an anaphylactic shock occurred in the operating room, enabling the scientists to identify the alternative IgG-dependent mechanism. They demonstrated that IgG antibodies activate neutrophils (50-70% of our white blood cells), releasing high doses of harmful vasodilating mediators. Neutrophil activation was more pronounced in cases of severe shock than in cases of moderate shock. Interestingly, the IgG-neutrophil pathway was also identified in most cases of shock where the traditional IgE-dependent mechanism was observed, suggesting that IgGs and neutrophils may contribute to the severity of most cases of shock via an additive effect.
“These findings elucidate 10 to 20% of cases of anaphylactic shock that previously had no biological explanation. They will be extremely valuable in refining diagnosis in these patients and avoiding any future exposure with the drug that triggered the allergic reaction,” explains Professor Sylvie Chollet-Martin (Paris-Sud University), joint last author of the study and Head of the Immunology laboratory on Autoimmunity and Hypersensitivity at Bichat Hospital.
This research was funded by the organizations mentioned in the first paragraph and by the European Research Council (ERC – FP7; MyeloSHOCK project).
 Mouse and Human Neutrophils induce Anaphylaxis, The Journal of Clinical Investigation, March 23, 2011. doi: 10.1172/JCI45232
Des recherches démontrent dans un modèle de souris au foie humanisé, que le VHD est capable in vitro et in vivo d'utiliser l'enveloppe d'autres virus que celle du VHB, comme celle du virus de l'hépatite C et de virus d'autres genres, comme ...
The World Health Organization (WHO) recommends a single dose of the yellow fever vaccine for individuals aged 9 months or older living in or traveling to areas at risk of disease transmission, but there is a lack of data on its long-term efficacy ...
An IgG-induced neutrophil activation pathway contributes to human drug-induced anaphylaxis, Science Translational Medicine, July 10, 2019
Friederike Jönsson1,†, Luc de Chaisemartin2,3,†, Vanessa Granger2,3,‡ , Aurélie Gouel-Chéron1,4,‡, Caitlin M. Gillis1, Qianqian Zhu1,3, Fadia Dib5, Pascale Nicaise-Roland2, Christelle Ganneau6, Maria Hurtado-Nedelec7, Catherine Paugam-Burtz8,9, Skander Necib8, Hawa Keita-Meyer9, Matthieu Le Dorze10, Bernard Cholley11, Olivier Langeron12, Laurent Jacob13, Benoit Plaud13, Marc Fischler14, Caroline Sauvan15, Marie-Thérèse Guinnepain16, Philippe Montravers4,17, Michel Aubier15,17, Sylvie Bay6, Catherine Neukirch15,17, NASA study group, Florence Tubach18,§ , Dan Longrois2,4,§, Sylvie Chollet-Martin2,3,§,* and Pierre Bruhns1,§,*
1Unit of Antibodies in Therapy and Pathology, Institut Pasteur, UMR1222 INSERM, F-75015 Paris, France.
2Département d'Immunologie et d'Hématologie, UF Auto-immunité et Hypersensibilités, HUPNVS, Hôpital Bichat, Paris, France.
3Inflammation Chimiokines et Immunopathologie, INSERM UMRS996, Faculté de Pharmacie, Université Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France.
4APHP, Hôpital Bichat, Département d'Anesthésie-Réanimation, HUPNVS, Paris, France.
5APHP, Hôpital Bichat, Department of Epidemiology and Clinical Research, INSERM, Paris, France.
6Unité de Chimie des Biomolécules, Institut Pasteur, UMR3523 CNRS, F-75015 Paris, France.
7Département d'Immunologie et d'Hématologie, UF Dysfonctionnements Immunitaires, HUPNVS, Hôpital Bichat, Paris, France.
8Département d'Anesthésie-Réanimation, Hôpital Beaujon, AP-HP, Clichy, France, and Université Paris Diderot, Paris, France.
9Service d’anesthésie, Hôpital Louis Mourier, AP-HP, Colombes, France and Université Paris Diderot, Sorbonne Paris Cité, EA Recherche Clinique coordonnée ville-hôpital, Méthodologies et Société (REMES), Paris, France.
10Département d'Anesthésie-Réanimation, Hôpital Lariboisière, AP-HP, Paris, France.
11Service d'Anesthésie-Réanimation, Hôpital Européen Georges Pompidou, AP-HP, UMR1140 INSERM, Paris, France and Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
12Department of Anesthesia and Critical Care, Hôpital Pitié Salpêtrière, AP-HP, Sorbonne Université, Paris, France and Infection and Epidemiology Department, Institut Pasteur Human Histopathology and Animal Models Unit, Paris, France
13Département d'Anesthésie-Réanimation, Hôpital Saint Louis, AP-HP, Paris, France.
14Service d'Anesthésie, Hôpital Foch, Suresnes, France.
15APHP, Hôpital Bichat, Service de Pneumologie A, HUPNVS, Paris, France.
16Hôpital Foch, Service de médecine interne, Suresnes, France.
17INSERM 1152, DHU FIRE, Labex Inflamex, Université Paris Diderot Paris 7, Paris, France.
18Sorbonne Université, INSERM, Institut Pierre Louis d’Epidémiologie et de Santé Publique, PEPITES, AP-HP, Hôpitaux Universitaires Pitié Salpêtrière - Charles Foix, Département Biostatistique Santé Publique et Information Médicale, Centre de Pharmacoépidémiologie (Cephepi), CIC-1421, F75013, Paris, France.
†, ‡: Equal contribution.
*: corresponding authors