Press releases

Association Between the Consumption of Food Additive Emulsifiers and the Risk of Cardiovascular Disease

07 Sep 2023 | By Inserm (Newsroom) | Public health

cœurThe scientists used the data reported by 95,442 French adults participating in the NutriNet-Santé study. © Mathilde Touvier/Inserm

Emulsifiers are among the additives most widely used by the food industry, helping to improve the texture of food and extend its shelf life. Researchers from Inserm, INRAE, Université Sorbonne Paris Nord, Université Paris Cité and Cnam, as part of the Nutritional epidemiology research team (EREN-CRESS), studied the impacts on cardiovascular health of the consumption of emulsifiers. They analyzed the health data of 95,442 adults participating in the French NutriNet-Santé cohort study in terms of their overall consumption of this type of food additive. The results suggest an association between the dietary intake of emulsifiers and an increased risk of cardiovascular disease. They have been published in the British Medical Journal.

In Europe and North America, 30 to 60% of dietary energy intake in adults is provided by ultra-processed foods. Recent epidemiological studies have linked high intakes of ultra-processed foods with higher risks of obesity, mortality and chronic diseases (cardiovascular disease, type 2 diabetes, cancer, etc.). Emulsifiers are among the most commonly used additives in industrial foods. They are often added to processed and packaged foods such as certain industrial cakes, cookies and desserts, as well as ice creams, candy bars, breads, margarines and ready meals, in order to improve their appearance, taste and texture and lengthen shelf life. These include celluloses, mono- and diglycerides of fatty acids, modified starches, lecithins, carrageenans, phosphates, gums and pectins.

As with all food additives, the safety of emulsifiers is regularly evaluated on the basis of the scientific evidence available at a given time. However, some recent studies suggest that emulsifiers can disrupt the gut microbiota and increase the risk of inflammation, leading to potentially increased susceptibility to cardiovascular problems.

In order to explore this question in greater depth, French researchers undertook to evaluate the links between exposure to emulsifiers and the risk of cardiovascular disease, including coronary heart- and cerebrovascular disease, i.e. conditions that affect blood circulation and blood vessels in the heart and brain.

Their conclusions are based on the analysis of data from 95,442 French adults (average age 43 years; 79% women) with no history of cardiovascular disease who voluntarily participated in the NutriNet-Santé cohort study (see box below) between 2009 and 2021.

During the first two years of follow-up, the participants completed at least three (and up to 21) days of online dietary records. Each food or drink consumed was then matched against databases to identify the presence and level of food additives, including emulsifiers. Laboratory assays were also performed in order to provide quantitative data.

The participants were asked to declare any major cardiovascular events, such as heart attack or stroke, which were validated by an expert committee following examination of their medical records. Deaths related to cardiovascular disease were also recorded using the French national registry of deaths.

Several well-known risk factors for heart disease, including age, sex, weight (BMI), educational level, family history, smoking and levels of physical activity, as well as overall quality of the diet (e.g. consumption of sugar, salt, energy and alcohol) were taken into account.

After an average follow-up of seven years, the scientists found that the higher intakes of total celluloses (food additive codes E460[1] to E468) were associated with higher risks of cardiovascular disease. In particular, this combination was specifically observed for intakes of E460 (microcrystalline cellulose, powdered cellulose) and E466 (carboxymethylcellulose).

On the other hand, higher intakes of mono- and diglycerides of fatty acids (E471 and E472) were associated with higher risks for all the diseases studied. Among these emulsifiers, lactic ester of mono- and diglycerides of fatty acids (E472b) was associated with a higher risk of cardiovascular and cerebrovascular disease, and citric acid ester of mono- and diglycerides of fatty acids (E472c) was associated with a higher risk of cardiovascular- and coronary heart disease.

High intake of trisodium phosphate (E339) was also associated with an increased risk of coronary heart disease.

No association was detected in this study between the other emulsifiers and the development of cardiovascular diseases.

Given its observational nature, the study cannot therefore establish causality on its own, and the scientists acknowledge it to have certain limitations. For example, the high proportion of women, the higher educational background and overall more health-conscious behaviors among the NutriNet-Santé study participants compared with the general French population, which may limit the generalizability of the results.

That being said, the study sample was large and the authors were able to consider a wide range of potentially confounding factors, while using detailed and unique data on food additives, down to the brand of the products consumed. In addition, the results remained unchanged after multiple sensitivity analyses, thereby strengthening their robustness.

“While these findings need to be replicated in other studies worldwide, they bring new key knowledge to the debate on re-evaluating the regulations around the use of additives in the food industry, in order to better protect consumers,” explains Mathilde Touvier, Research Director at Inserm, and Bernard Srour, Junior Professor at INRAE, principal authors of the study.

NutriNet-Santé is a public health study coordinated by the Nutritional epidemiology research team (EREN-CRESS, Inserm/INRAE/Cnam/Université Sorbonne Paris Nord/Université Paris Cité) which, thanks to the commitment and loyalty of over 170,000 participants (known as Nutrinautes), advances research into the links between nutrition (diet, physical activity, nutritional status) and health. Launched in 2009, the study has already given rise to over 270 international scientific publications. In France, a drive to recruit new participants is still ongoing in order to continue to further research into the relationship between nutrition and health.

By devoting a few minutes per month to answering questionnaires on diet, physical activity and health through the secure online platform, participants in France contribute to furthering knowledge of the links between diet and health.

[1] Food additives are identified in the list of ingredients by a European code consisting of the letter “E”, followed by a number making it possible to easily identify the category. For example, E100 for colorants, E200 for preservatives, E400 for emulsifiers and texture agents.

Researcher Contact

Mathilde Touvier

Inserm Research Director

Leader of the Nutritional epidemiology research team (EREN), unit 1153 Inserm/INRAE/Cnam/Université Sorbonne Paris Nord, Center of Research in Epidemiology and Statistics (CRESS)



Dr Bernard Srour

INRAE Junior Professor

Nutritional epidemiology research team (EREN), unit 1153 Inserm/INRAE/Cnam/Université Sorbonne Paris Nord, Université Paris Cité, Center of Research in Epidemiology and Statistics (CRESS)


Press Contact



Food additive emulsifiers and risk of cardiovascular disease in the NutriNet-Santé cohort: prospective cohort study

Laury Sellem1, Bernard Srour1, Guillaume Javaux1, Eloi Chazelas1, Benoit Chassaing2, Emilie Viennois3 Charlotte Debras,1 Clara Salame,1 Nathalie Druesne-Pecollo1, Younes Esseddik1, Fabien Szabo de Edelenyi1, Cedric Agaesse,1 Alexandre De Sa1, Rebecca Lutchia1, Erwan Louveau1, Inge Huybrechts4, Fabrice Pierre5, Xavier Coumoul6, Leopold K Fezeu1, Chantal Julia1,7, Emmanuelle Kesse-Guyot1, Benjamin Alles1, Pilar Galan1, Serge Hercberg,1,7, Melanie Deschasaux-Tanguy1, Mathilde Touvier1

1 Université Sorbonne Paris Nord and Université Paris Cite, Inserm, INRAE, CNAM, Center of Research in Epidemiology and StatisticS (CRESS), Nutritional Epidemiology Research Team (EREN), Bobigny, France

2 Inserm U1016, team “Mucosal microbiota in chronic inflammatory diseases,” Universite Paris Cite, Paris, France

3 Inserm U1149, Centre for Research on Inflammation, Université de Paris, Paris, France

4 International Agency for Research on Cancer, World Health Organization, Lyon, France

5Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRAE, ENVT, INP-Purpan, UPS, Toulouse, France

6 Inserm UMR-S 1124, Universite Paris Cite, Paris, France

British Medical Journal, le 7 septembre 2023