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Segmented filamentous bacteria, partners in intestinal immunity, finally cultured in vitro !

Segmented filamentous bacteria (SFB) are bacteria from the Clostridiaceae family that colonize the intestines of many species, likely including humans, without causing disease; they live in symbiosis with epithelial cells and are involved in the maturation of intestinal immunity.

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©Inserm/Martin, Sandra

Despite the vital role of SFB in establishing gut immune homeostasis in vivo in mice, scientists, for the past 50 years, have been unable to reproduce this symbiosis in vitro to shed light on the cellular and molecular interactions involved. Now, scientists from the Institut Pasteur, the Collège de France, Inserm/Imagine-Necker and the Université Paris Descartes have successfully cultured and reproduced the complex life-cycle of these bacteria outside their host for the first time.

A closer look at the symbiosis between SFB and their host

A single-celled intracellular offspring form of these bacteria attach themselves directly to intestinal epithelial cells, creating a mutually beneficial interaction between the bacteria and the host.

This non-pathogenic yet intimate relationship, which is established immediately after weaning, provides the bacteria with nutrients and, in turn, stimulates the development and maturation of the intestinal immune system. The delicate balance between these bacteria and their host protects the host from pathogens by strengthening both the epithelial barrier and the immune system. Animals lacking segmented filamentous bacteria in their intestinal flora are more susceptible to infection, even in adulthood.

The reproduction of this complex system in vitro should finally enable scientists to decode the dialog between the host and the bacteria and also to shed light on the way in which antibiotics can disrupt immune development as antibiotics lead to SFB disappearance from the gut.

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Growth and host interaction of mouse Segmented Filamentous Bacteria in vitro, Nature, xx décembre 2014

  • Pamela Schnupf1,3, Valérie Gaboriau-Routhiau3,4,5, Marine Gros5,6, Robin Friedman1, Maryse Moya-Nilges2, Giulia Nigro1, Nadine Cerf-Bensussan3,5*, Philippe Sansonetti1,7*
  • 1Unité de Pathogénie Microbienne Moleculaire, INSERM, U786
  • 2Imagopole, Ultrastructural Microscopy Platform, Institut Pasteur, 25–28 Rue du Dr Roux, 75724 Paris Cedex 15, France
  • 3INSERM, UMR1163, Laboratory of Intestinal Immunity
  • 4INRA Micalis UMR1319, 78350 Jouy-en-Josas, France
  • 5Université Paris Descartes-Sorbonne Paris Cité and Institut Imagine, 75015 Paris, France
  • 6Ecole Normale Superieure de Lyon, 69007 Lyon, France
  • 7Microbiologie et Maladies Infectieuses, Collège de France, Paris, France