Press releases

Identified: A Protein Essential for Chikungunya Virus Replication

Press release | 25 Sep 2019 - 19h00 | By INSERM PRESS OFFICE
Immunology, inflammation, infectiology and microbiology

Fibroblast infected with the chikungunya virus. © Inserm/Thérèse Couderc/Marie-Christine Prévost/Marc Lecuit

Chikungunya is an infectious disease caused by a mosquito-borne virus transmitted to humans. First detected in Africa, the virus has been responsible for recent epidemics in the Americas, Asia and the Indian Ocean – particularly the Reunion Island. Chikungunya is characterized by high fever and intense joint and muscle pain that can last for several months. The mechanisms of infection of human cells with the virus remain very poorly understood. Led by Ali Amara in collaboration with Marc Lecuit researchers from Inserm, Institut Pasteur, CNRS and Université de Paris have identified a protein that is crucial in order for the virus to replicate within its target cells. This research, published in the journal Nature, opens up therapeutic avenues in the fight against chikungunya.

Originally from Africa, chikungunya is aptly named. It derives from a word in the Kimakonde language, meaning “to become contorted”, because the severe muscle and joint pains endured by the patients prevent them from moving normally or performing their daily activities.

While the clinical manifestations of the disease are well understood, the mechanisms by which the virus infects human cells and multiplies remain poorly elucidated. Several studies had already identified certain host cell factors implicated in the replication of the virus. However, none had succeeded in explaining why the virus preferentially targets the muscle and joint cells, causing these clinical signs.

Researchers from Inserm, CNRS and Université de Paris led by Dr. Ali Amara at the AP-HP Saint-Louis Hospital Research Institute in Paris, in collaboration with Marc Lecuit’s team from Institut Pasteur, Inserm and Université de Paris, have identified that the FHL1 protein is a key cellular factor for the replication and pathogenesis of chikungunya. FHL1 is a molecule present mainly in the muscle cells and fibroblasts, the preferred targets of the virus. Usually, FHL1 contributes to healthy muscle physiology and it is now thought to be diverted from that function by the virus to ensure its replication in the target cells.

To conduct this study, Amara’s team used the CRISPR-Cas9 technology to systematically screen the genome of human cells in order to identify the host factors necessary for viral replication. In doing so, it isolated the gene coding for the FHL1 protein. The team then conducted a series of experiments showing the incapacity of the virus to infect cells whose FHL1 expression had been abolished.

In addition, the researchers have shown that the virus was incapable of multiplying within cells derived from patients suffering Emery-Dreifuss muscular dystrophy – a rare genetic disease. This muscle disease is the result of mutations of the FHL1 gene responsible for the breakdown of the FHL1 protein. The researchers have shown that the cells of these patients are resistant to the virus.

Finally, the researchers performed in vivo experiments in mice whose Fhl1 gene was invalidated. They have shown that these animals are totally resistant to infection and do not develop the disease, whereas the virus multiplies and causes major muscle lesions in mice expressing a functional FHL1 protein. These observations demonstrate that the FHL1 protein plays a key role in chikungunya virus replication and pathogenesis.

The precise role played by FHL1 in the viral infection is not fully understood. The researchers have discovered that FHL1 interacts with a viral protein known as nsP3. It is when binding to this that FHL1 participates in the replication of the virus.

“We now want to understand this interaction in molecular detail. The next step is to define why FHL1 is so specific to the chikungunya virus, and to decipher its mechanism of action at the molecular level. Elucidating the molecular structure of the FHL1-nsP3 complex could represent a major step forward in the development of antivirals that block the replication of the virus“, emphasize Ali Amara and Laurent Meertens, the Inserm researchers in charge of the study.

At present, only symptomatic treatments are available for patients infected with chikungunya.

TO CITE THIS POST :
Press release – Inserm press room Identified: A Protein Essential for Chikungunya Virus Replication Link : https://presse.inserm.fr/en/identified-a-protein-essential-for-chikungunya-virus-replication/36716/
Medias
Researcher Contact

Ali Amara

Group Leader, Inserm Junior Research Director, accreditation to supervise research

Unit 944 “Genomes & Cell Biology of Disease” (Inserm, CNRS, Université Paris Diderot – Paris 7)

Tel.:     +33 (0)1 53 72 40 60

Email: ali.amara@inserm.fr

 

Laurent Meertens

Inserm Senior Research Associate

Unit 944 “Genomes & Cell Biology of Disease” (Inserm, CNRS, Université Paris Diderot – Paris 7)

Tel.:     +33 (0)1 53 72 40 62

Email: laurent.meertens@inserm.fr

Press Contact

presse@inserm.fr

Sources

FHL1 is a major host factor for chikungunya virus infection

Laurent Meertens1,13*, Mohamed Lamine Hafirassou1, Therese Couderc2, Lucie Bonnet-Madin1, Vasiliya Kril1,  Beate M. Kümmerer3, Athena Labeau1, Alexis Brugier1, Etienne Simon-Loriere4, Julien Burlaud-Gaillard5, Cécile Doyen6,  Laura Pezzi7, Thibaud Goupil2, Sophia Rafasse2, Pierre-Olivier Vidalain8, Anne Bertrand Legout9, Lucie Gueneau9,  Raul Juntas-Morales10, Rabah Ben Yaou9, Gisèle Bonne9, Xavier de Lamballerie7, Monsef Benkirane6, Philippe Roingeard5, Constance Delaugerre1,11, Marc Lecuit2,12 & Ali Amara1,13*

 

1 Inserm U944, CNRS UMR 7212, Genomes & Cell Biology of disease Unit Institut de Recherche Saint-Louis, Université de Paris, Hôpital Saint-Louis Paris France

2 Institut Pasteur, Inserm U1117, Biology of Infection Unit Paris France

3 Institute of Virology University of Bonn Medical Centre Bonn Germany

4 Institut Pasteur, G5 Evolutionary Genomics of RNA Viruses Paris France

5 Inserm U1259 MAVIVH et Plateforme IBiSA de Microscopie Electronique Université de Tours et CHRU de Tours Tours France

6 Institut de Génétique Humaine, Laboratoire de Virologie Moléculaire CNRS-Université de Montpellier Montpellier France

7 Unité des Virus Émergents, Aix-Marseille Univ, IRD190, Inserm 1207, EFS-IRBA Marseille France

8 Equipe Chimie & Biologie, Modélisation et Immunologie pour la Thérapie Université Paris Descartes, CNRS UMR 8601 Paris France

9 Center of Research in Myology, Sorbonne Université, Inserm UMRS974 Paris France

10 Département de Neurologie Centre Hospitalier Universitaire de Montpellier Montpellier France

11 Laboratoire de Virologie et Département des Maladies Infectieuses Hôpital Saint-Louis, APHP Paris France

12 Université de Paris, Department of Infectious Diseases and Tropical Medicine Necker-Enfants Malades University Hospital, APHP, Institut Imagine Paris France

13* Corresponding authors

 

Nature, Septembre 2019

DOI : https://doi.org/10.1038/s41586-019-1578-4

fermer
fermer
RSS Youtube